{Reference Type}: Journal Article
{Title}: Transcriptional, post-transcriptional, and post-translational regulation of polyunsaturated fatty acid synthase genes in Aurantiochytrium limacinum strain BL10: Responses to nitrogen starvation.
{Author}: Wu YC;Liu T;Liu CN;Kuo CY;Ting YH;Wu CA;Shen XL;Wang HC;Chen CJ;Renta PP;Chen YL;Hung MC;Chen YM;
{Journal}: Int J Biol Macromol
{Volume}: 274
{Issue}: 0
{Year}: 2024 Aug 15
{Factor}: 8.025
{DOI}: 10.1016/j.ijbiomac.2024.133177
{Abstract}: Under nitrogen deficient conditions, the Aurantiochytrium limacinum strain BL10 greatly increases the production of docosahexaenoic acid (DHA) and n-6 docosapentaenoic acid. Researchers have yet to elucidate the mechanism by which BL10 promotes the activity of polyunsaturated fatty acid synthase (Pfa), which plays a key role in the synthesis of polyunsaturated fatty acid (PUFA). Analysis in the current study revealed that in nitrogen-depleted environments, BL10 boosts the transcription and synthesis of proteins by facilitating the expression of pfa genes via transcriptional regulation. It was also determined that BL10 adjusts the lengths of the 5'- and 3'-untranslated regions (suggesting post-transcriptional regulation) and modifies the ratio of two Pfa1 isoforms to favor PUFA production via post-translational regulation (ubiquitination). These findings clarify the exceptional DHA production of BL10 and provide additional insights into the regulatory mechanisms of PUFA biosynthesis in Aurantiochytrium.