{Reference Type}: Journal Article
{Title}: Development and Clinical Evaluation of a CRISPR/Cas12a-Based Nucleic Acid Detection Platform for the Diagnosis of Keratomycoses.
{Author}: Deivarajan HR;Elamurugan V;Sivashanmugam P;Pandian J;Sevugamurthi K;Rameshkumar G;Ghosh S;Banerjee D;Venugopal A;Jose A;Rammohan R;Raghavan A;Rajaraman R;Kuppamuthu D;Prajna L;Prajna VN;Narendran S;
{Journal}: Ophthalmol Sci
{Volume}: 4
{Issue}: 5
{Year}: 2024 Sep-Oct
暂无{DOI}: 10.1016/j.xops.2024.100522
{Abstract}: <B>UNASSIGNED: </B>The objective of this study was to develop a rapid and accurate clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a-based molecular diagnostic assay (Rapid Identification of Mycoses using CRISPR, RID-MyC assay) to detect fungal nucleic acids and to compare it with existing conventional mycologic methods for the diagnosis of fungal keratitis (FK).<BR><B>UNASSIGNED: </B>This study was structured as a development and validation study focusing on the creation and assessment of the RID-MyC assay as a novel diagnostic modality for FK.<BR><B>UNASSIGNED: </B>Participants comprised 142 individuals presenting with suspected microbial keratitis at 3 tertiary care institutions in South India.<BR><B>UNASSIGNED: </B>The RID-MyC assay utilized recombinase polymerase amplification targeting the 18S ribosomal RNA gene for isothermal amplification, followed by a CRISPR/Cas12a reaction. This was benchmarked against microscopy, culture, and polymerase chain reaction for the diagnosis of FK.<BR><B>UNASSIGNED: </B>The primary outcome measures focused on the analytical sensitivity and specificity of the RID-MyC assay in detecting fungal nucleic acids. Secondary outcomes measured the assay's diagnostic sensitivity and specificity for FK, including its concordance with conventional diagnostic methods.<BR><B>UNASSIGNED: </B>The RID-MyC assay exhibited a detection limit ranging from 13.3 to 16.6 genomic copies across 4 common fungal species. In patients with microbial keratitis, the RID-MyC assay showed substantial agreement with microscopy (kappa = 0.714) and fair agreement with culture (kappa = 0.399). The assay demonstrated a sensitivity of 93.27% (95% confidence interval [CI], 86.62%-97.25%) and a specificity of 89.47% (95% CI, 66.86%-98.70%) for FK diagnosis, with a median diagnostic time of 50 minutes (range, 35-124 minutes).<BR><B>UNASSIGNED: </B>The RID-MyC assay, utilizing CRISPR-Cas12a technology, offers high diagnostic accuracy for FK. Its potential for point-of-care use could expedite and enhance the precision of fungal diagnostics, presenting a promising solution to current diagnostic challenges.<BR><B>UNASSIGNED: </B>Proprietary or commercial disclosure may be found in the Footnotes and Disclosures at the end of this article.