{Reference Type}: Journal Article {Title}: A ratiometric fluorescent probe based on a novel fluorophore with high selectivity for imaging cysteine in living cells. {Author}: Miao Z;Li C;Pang S;Du C;Wei N;Zhang Y; {Journal}: Luminescence {Volume}: 39 {Issue}: 6 {Year}: 2024 Jun {Factor}: 2.613 {DOI}: 10.1002/bio.4806 {Abstract}: As a biothiol, cysteine (Cys) is essential to both physiological and pathological processes and has been associated with many diseases, including neurological disorders, rheumatoid arthritis, and renal dysfunction. Therefore, the development of a high-performance probe for detecting Cys levels can help prevent and diagnose disease. In this study, a ratiometric fluorescent probe based on a novel fluorophore was developed for detecting Cys, and it showed high specificity and a rapid response time toward Cys. This probe demonstrates excellent biocompatibility and has been utilized effectively for the imaging of Cys in living cells.