{Reference Type}: Journal Article
{Title}: Two point mutations N771S and K847N in the VHA-a of Phytophthora litchii confer resistance to fluopimomide.
{Author}: Dai T;Wang Z;Yang J;Yuan K;Miao J;Liu X;
{Journal}: Pestic Biochem Physiol
{Volume}: 202
{Issue}: 0
{Year}: 2024 Jun
{Factor}: 4.966
{DOI}: 10.1016/j.pestbp.2024.105900
{Abstract}: The phytopathogenic oomycete Phytophthora litchii is the culprit behind the devastating disease known as "litchi downy blight", which causes large losses in litchi production. Although fluopimomide exhibits strong inhibitory efficacy against P. litchii, the exact mechanism of resistance is still unknown. The sensitivity of 137 P. litchii isolates to fluopimomide was assessed, and it was discovered that the median effective concentration (EC50) of the fungicide had a unimodal frequency distribution with a mean value of 0.763 ± 0.922 μg/mL. Comparing the resistant mutants to the equivalent parental isolates, the resistance mutants' survival fitness was much lower. While there was no cross-resistance between fluopimomide and other oomycete inhibitors, there is a notable positive cross-resistance between fluopimomide and fluopicolide. According to the thorough investigation, P. litchii had a moderate chance of developing fluopimomide resistance. The point mutations N771S and K847N in the VHA-a of P. litchii (PlVHA-a) were present in the fluopimomide-resistant mutants, and the two point mutations in PlVHA-a conferring fluopimomide resistance were verified by site-directed mutagenesis in the sensitive P. capsici isolate BYA5 and molecular docking.