{Reference Type}: Journal Article {Title}: Seasonal changes in hydration in free-living Japanese children and adolescents. {Author}: Amano T;Sato K;Otsuka J;Okamoto Y;Takada S;Kato H;Yokoyama S;Oshima S;Hosokawa Y;Fujii N;Mündel T;Kenny G;Hiwa T;Inoue Y; {Journal}: Appl Physiol Nutr Metab {Volume}: 0 {Issue}: 0 {Year}: 2024 Jun 14 {Factor}: 3.016 {DOI}: 10.1139/apnm-2023-0464 {Abstract}: Changes in hydration status occur throughout the day affecting physiological and behavioural functions. However, little is known about the hydration status of free-living Japanese children and the seasonality of this response. We evaluated hydration status estimated by urine osmolality (Uosm) in 349 children (189 boys and 160 girls, 9.5 ± 2.6 years, range: 6-15 years) upon waking at home and during a single school day in spring (April) and summer (July). Further, we assessed the efficacy of employing self-assessment of urine colour (UC; based on an 8-point scale) by children to monitor their hydration status. Early morning Uosm was greater in the spring (903 ± 220 mOsm L-1; n = 326) as compared to summer (800 ± 244 mOsm L-1; n = 125) (P = 0.003, paired t test, n = 104). No differences, however, were observed in Uosm during the school day (P = 0.417, paired t test, n = 32). While 66% and 50% of children were considered underhydrated (Uosm ≥ 800 mOsm L-1) upon waking in the spring and summer periods, respectively, more children were underhydrated (∼12%) during the school day. Self-reported UC was similar between seasons as assessed in the morning and school day (P ≥ 0.101, paired t test), which differed from the pattern of responses observed with Uosm. We showed that a significant number of Japanese children are likely underhydrated especially in the spring period. Children do not detect seasonal changes in hydration from self-assessed UC, limiting its utility to manage hydration status in children.