{Reference Type}: Journal Article
{Title}: Extended depth-of-field wide-field fluorescence microscopy with a micro-mirror array lens system for versatile cellular examination.
{Author}: Jeon S;Lee J;Kim K;Hong SM;Oh BH;Kim KH;
{Journal}: Opt Lett
{Volume}: 49
{Issue}: 12
{Year}: 2024 Jun 15
{Factor}: 3.56
{DOI}: 10.1364/OL.515783
{Abstract}: We present a versatile extended depth-of-field (EDOF) wide-field fluorescence microscopy using a new, to the best of our knowledge, active device, micro-mirror array lens system (MALS) for calibration-free and orientation-insensitive EDOF imaging. The MALS changed the focal plane during image acquisition, and the system could be operated in any orientation. Two EDOF imaging modes of high-speed accumulation and low-speed surface sectioning were implemented. The performance was demonstrated in non-contact imaging of conjunctival goblet cells in live mice and depth-resolved cellular examination of ex-vivo human cancer specimens. MALS-based EDOF microscopy has potential for versatile cellular examination.