{Reference Type}: Journal Article
{Title}: Protocol for whole-mount immunofluorescence staining of ECM gel-embedded innervated pancreatic organoids.
{Author}: Beşikcioğlu HE;Yurteri Ü;Ye L;Zhang F;Moretti A;Gürcinar IH;Dogruöz A;Karakas D;Friess H;Ceyhan GO;Istvanffy R;Ekin Demir I;
{Journal}: STAR Protoc
{Volume}: 5
{Issue}: 2
{Year}: 2024 Jun 21
暂无{DOI}: 10.1016/j.xpro.2024.103132
{Abstract}: The mandatory usage of extracellular matrix (ECM) gels in 3D cultures limits antibody penetration and increases background, while the removal of ECM gel causes disruption of morphology and sample loss. These factors pose challenges to effective immune labeling-based staining. Here, we present a protocol for whole-mount immunofluorescence staining of gel-embedded pancreatic organoids. We describe steps for sample fixation, blocking, and antibody incubation. We detail procedures for washing antibodies and mounting.