{Reference Type}: Journal Article
{Title}: A Two-color Single-molecule Sequencing Platform and Its Clinical Applications.
{Author}: Chen F;Liu B;Chen M;Jiang Z;Zhou Z;Wu P;Zhang M;Jin H;Li L;Lu L;Shang H;Liu L;Chen W;Xu J;Sun R;Wang G;Zheng J;Qi J;Yang B;Zeng L;Li Y;Lv H;Zhao N;Wang W;Cai J;Liu Y;Luo W;Zhang J;Zhang Y;Fan J;Dan H;He X;Huang W;Sun L;Yan Q;
{Journal}: Genomics Proteomics Bioinformatics
{Volume}: 22
{Issue}: 1
{Year}: 2024 05 9
{Factor}: 6.409
{DOI}: 10.1093/gpbjnl/qzae006
{Abstract}: DNA sequencers have become increasingly important research and diagnostic tools over the past 20 years. In this study, we developed a single-molecule desktop sequencer, GenoCare 1600 (GenoCare), which utilizes amplification-free library preparation and two-color sequencing-by-synthesis chemistry, making it more user-friendly compared with previous single-molecule sequencing platforms for clinical use. Using the GenoCare platform, we sequenced an Escherichia coli standard sample and achieved a consensus accuracy exceeding 99.99%. We also evaluated the sequencing performance of this platform in microbial mixtures and coronavirus disease 2019 (COVID-19) samples from throat swabs. Our findings indicate that the GenoCare platform allows for microbial quantitation, sensitive identification of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, and accurate detection of virus mutations, as confirmed by Sanger sequencing, demonstrating its remarkable potential in clinical application.