{Reference Type}: Journal Article
{Title}: Using cerebrospinal fluid nanopore sequencing assay to diagnose tuberculous meningitis: a retrospective cohort study in China.
{Author}: Zhou L;Zou X;Yong Y;Hu Q;
{Journal}: BMJ Open
{Volume}: 14
{Issue}: 6
{Year}: 2024 Jun 11
{Factor}: 3.006
{DOI}: 10.1136/bmjopen-2023-080904
{Abstract}: <B>OBJECTIVE: </B>This study aimed to evaluate the efficiency of nanopore sequencing for the early diagnosis of tuberculous meningitis (TBM) using cerebrospinal fluid and compared it with acid-fast bacilli (AFB) smear, mycobacterial growth indicator tube culture and Xpert Mycobacterium tuberculosis (MTB)/rifampicin (RIF).<BR><B>METHODS: </B>Single-centre retrospective study.<BR><B>METHODS: </B>The Tuberculosis Diagnosis and Treatment Center of Zhejiang Chinese and Western Medicine Integrated Hospital.<BR><B>METHODS: </B>We enrolled 64 adult patients with presumptive TBM admitted to our hospital from August 2021 to August 2023.<BR><B>METHODS: </B>We calculated the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of AFB smear, culture, Xpert MTB/RIF and nanopore sequencing to evaluate their diagnostic efficacy compared with a composite reference standard for TBM.<BR><B>RESULTS: </B>Among these 64 patients, all tested negative for TBM by AFB smear. The sensitivity, specificity, PPV and NPV were 11.11%, 100%, 100% and 32.2% for culture, 13.33%, 100%, 100% and 2.76% for Xpert MTB/RIF, and 77.78%, 100%, 100% and 65.52% for nanopore sequencing, respectively.<BR><B>CONCLUSIONS: </B>The diagnostic accuracy of the nanopore sequencing test was significantly higher than that of conventional testing methods used to detect TBM.