{Reference Type}: Journal Article
{Title}: Selection of structure-induced aptamer targeting small molecule based on capillary sieving electrophoresis.
{Author}: Wada M;Endo T;Hisamoto H;Sueyoshi K;
{Journal}: Anal Sci
{Volume}: 0
{Issue}: 0
{Year}: 2024 Jun 11
{Factor}: 1.967
{DOI}: 10.1007/s44211-024-00588-6
{Abstract}: In this study, a structure-induced aptamer targeting small molecules was selected using capillary sieving electrophoresis (CSE). CSE was conducted using a capillary filled with a background solution containing hydroxypropyl cellulose as a sieving matrix to separate the aptamer candidates by changing their structures via complexation. Before aptamer selection, the original random-sequence DNA library was used to create structure-not-preorganized DNA sub-library containing straight-chain-like structures using CSE. Next, a structure-induced aptamer targeting L-tyrosinamide was selected from the prepared sub-library. Six aptamer candidates were selected, one of which showed a binding ability comparable to that of the reported L-tyrosinamide aptamer and selectivity toward the analogs. These results indicated that the proposed method can be applied to select structure-induced aptamers that target small molecules.