{Reference Type}: Journal Article
{Title}: Determination of Phosphoglycolate Phosphatase Activity via a Coupled Reaction Using Recombinant Glycolate Oxidase.
{Author}: Duminil P;Oury C;Hodges M;Glab N;
{Journal}: Methods Mol Biol
{Volume}: 2792
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3802-6_3
{Abstract}: Phosphoglycolate phosphatase (PGLP) dephosphorylates 2-phosphoglycolate to glycolate that can be further metabolized to glyoxylate by glycolate oxidase (GOX) via an oxidative reaction that uses O2 and releases H2O2. The oxidation of o-dianisidine by H2O2 catalyzed by a peroxidase can be followed in real time by an absorbance change at 440 nm. Based on these reactions, a spectrophotometric method for measuring PGLP activity using a coupled reaction with recombinant Arabidopsis thaliana GOX is described. This protocol has been used successfully with either purified PGLP or total soluble proteins extracted from Arabidopsis rosette leaves.