{Reference Type}: Journal Article
{Title}: Multiplexed Detection Strategies for Biosensors Based on the CRISPR-Cas System.
{Author}: Wei C;Lei X;Yu S;
{Journal}: ACS Synth Biol
{Volume}: 13
{Issue}: 6
{Year}: 2024 Jun 21
{Factor}: 5.249
{DOI}: 10.1021/acssynbio.4c00161
{Abstract}: A growing number of applications require simultaneous detection of multiplexed nucleic acid targets in a single reaction, which enables higher information density in combination with reduced assay time and cost. Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-Cas system have broad applications for the detection of nucleic acids due to their strong specificity, high sensitivity, and excellent programmability. However, realizing multiplexed detection is still challenging for the CRISPR-Cas system due to the nonspecific collateral cleavage activity, limited signal reporting strategies, and possible cross-reactions. In this review, we summarize the principles, strategies, and features of multiplexed detection based on the CRISPR-Cas system and further discuss the challenges and perspective.