{Reference Type}: Journal Article
{Title}: Homonuclear Simplified Preservation of Equivalent Pathways Spectroscopy.
{Author}: Nimerovsky E;Kosteletos S;Lange S;Becker S;Lange A;Andreas LB;
{Journal}: J Phys Chem Lett
{Volume}: 15
{Issue}: 24
{Year}: 2024 Jun 20
{Factor}: 6.888
{DOI}: 10.1021/acs.jpclett.4c00991
{Abstract}: Recently developed homonuclear transverse mixing optimal control pulses (hTROP) revealed an elegant way to enhance the detected signal in multidimensional magic-angle spinning (MAS) nuclear magnetic resonance experiments. Inspired by their work, we present two homonuclear simplified preservation of equivalent pathways spectroscopy (hSPEPS) sequences for recoupling CA-CO and CA-CB dipolar couplings under fast and ultrafast MAS rates, theoretically enabling a √2 improvement in sensitivity for each indirect dimension. The efficiencies of hSPEPS are evaluated for non-deuterated samples of influenza A M2 and bacterial rhomboid protease GlpG under two different external magnetic fields (600 and 1200 MHz) and MAS rates (55 and 100 kHz). Three-dimensional (H)CA(CO)NH, (H)CO(CA)NH, and (H)CB(CA)NH spectra demonstrate the high robustness of hSPEPS elements to excite carbon-carbon correlations, especially in the (H)CB(CA)NH spectrum, where hSPEPS outperforms the J-based sequence by a factor of, on average, 2.85.