{Reference Type}: Journal Article
{Title}: The Pre- and Post-column Derivatization on Monosaccharide Composition Analysis, a Review.
{Author}: Chen X;Wang Y;Ye Y;Yu H;Wu B;
{Journal}: Chem Biodivers
{Volume}: 0
{Issue}: 0
{Year}: 2024 Jun 10
{Factor}: 2.745
{DOI}: 10.1002/cbdv.202400749
{Abstract}: Polysaccharides, as common metabolic products in organisms, play a crucial role in the growth and development of living organisms. For humans, polysaccharides represent a class of compounds with diverse applications, particularly in the medical field. Therefore, the exploration of the monosaccharide composition and structural characteristics of polysaccharides holds significant importance in understanding their biological functions. This review provides a comprehensive overview of extraction methods and hydrolysis strategies for polysaccharides. It systematically analyzes strategies and technologies for determining polysaccharide composition and discusses common derivatization reagents employed in further polysaccharide studies. Derivatization is considered a fundamental strategy for determining monosaccharides, as it not only enhances the detectability of analytes but also increases detection sensitivity, especially in liquid chromatography (LC), capillary electrophoresis (CE), and gas chromatography (GC) techniques. The critical comparison of pre- and post-column derivatization is elaborated in this paper, aiming to serve as a reference for selecting appropriate modes based on the structural characteristics, biological activities of polysaccharides, and reaction systems.