{Reference Type}: Journal Article
{Title}: The CssRS two-component system of Bacillus subtilis contributes to teicoplanin and polymyxin B response.
{Author}: Kachan AV;Evtushenkov AN;
{Journal}: Folia Microbiol (Praha)
{Volume}: 0
{Issue}: 0
{Year}: 2024 Jun 7
{Factor}: 2.629
{DOI}: 10.1007/s12223-024-01179-8
{Abstract}: CssRS is a two-component system that plays a pivotal role in mediating the secretion stress response in Bacillus subtilis. This system upregulates the synthesis of membrane-bound HtrA family proteases that cope with misfolded proteins that accumulate within the cell envelope as a result of overexpression or heat shock. Recent studies have shown the induction of CssRS-regulated genes in response to cell envelope stress. We investigated the induction of the CssRS-regulated htrA promoter in the presence of different cell wall- and membrane-active substances and observed induction of the CssRS-controlled genes by glycopeptides (vancomycin and teicoplanin), polymyxins B and E, certain β-lactams, and detergents. Teicoplanin was shown to elicit remarkably stronger induction than vancomycin and polymyxin B. Teicoplanin and polymyxin B induced the spxO gene expression in a CssRS-dependent fashion, resulting in increased activity of Spx, a master regulator of disulfide stress in Bacillus subtilis. The CssRS signaling pathway and Spx activity were demonstrated to be involved in Bacillus subtilis resistance to teicoplanin and polymyxin B.