{Reference Type}: Journal Article {Title}: Effect of locomotor preference on the evolution of mitochondrial genes in Bovidae. {Author}: Shi L;Wang X;Yang X;Lyu T;Wang L;Zhou S;Dong Y;Wu X;Shang Y;Zhang H; {Journal}: Sci Rep {Volume}: 14 {Issue}: 1 {Year}: 2024 06 5 {Factor}: 4.996 {DOI}: 10.1038/s41598-024-63937-5 {Abstract}: Locomotor preferences and habitat types may drive animal evolution. In this study, we speculated that locomotor preference and habitat type may have diverse influences on Bovidae mitochondrial genes. We used selection pressure and statistical analysis to explore the evolution of mitochondrial DNA (mtDNA) protein-coding genes (PCGs) from diverse locomotor preferences and habitat types. Our study demonstrates that locomotor preference (energy demand) drives the evolution of Bovidae in mtDNA PCGs. The habitat types had no significant effect on the rate of evolution in Bovidae mitochondrial genes. Our study provides deep insight into the adaptation of Bovidae.