{Reference Type}: Journal Article
{Title}: A real-time biochemical assay for quantitative analyses of APOBEC-catalyzed DNA deamination.
{Author}: Belica CA;Carpenter MA;Chen Y;Brown WL;Moeller NH;Boylan IT;Harris RS;Aihara H;
{Journal}: J Biol Chem
{Volume}: 300
{Issue}: 6
{Year}: 2024 Jun 23
暂无{DOI}: 10.1016/j.jbc.2024.107410
{Abstract}: Over the past decade, the connection between APOBEC3 cytosine deaminases and cancer mutagenesis has become increasingly apparent. This growing awareness has created a need for biochemical tools that can be used to identify and characterize potential inhibitors of this enzyme family. In response to this challenge, we have developed a Real-time APOBEC3-mediated DNA Deamination assay. This assay offers a single-step set-up and real-time fluorescent read-out, and it is capable of providing insights into enzyme kinetics. The assay also offers a high-sensitivity and easily scalable method for identifying APOBEC3 inhibitors. This assay serves as a crucial addition to the existing APOBEC3 biochemical and cellular toolkit and possesses the versatility to be readily adapted into a high-throughput format for inhibitor discovery.