{Reference Type}: Journal Article
{Title}: Characterization of a novel inulosucrase from Lactiplantibacillus plantarum.
{Author}: Sarang S;Ernst L;Wefers D;Kulkarni R;
{Journal}: Food Chem
{Volume}: 453
{Issue}: 0
{Year}: 2024 Sep 30
{Factor}: 9.231
{DOI}: 10.1016/j.foodchem.2024.139597
{Abstract}: Fructansucrases produce fructans by polymerizing the fructose moiety released from sucrose. Here, we describe the recombinant expression and characterization of a unique fructansucrase from Lactiplantibacillus plantarum DKL3 that showed low sequence similarity with previously characterized fructansucrases. The optimum pH and temperature of fructansucrase were found to be 4.0 and 35 °C, respectively. Enzyme activity increased in presence of Ca2+ and distinctly in presence of Mn2+. The enzyme was characterized as an inulosucrase (LpInu), based on the production of an inulin-type fructan as assessed byNMR spectroscopy and methylation analysis. In addition to β-2,1-linkages, the inulin contained a few β-2,1,6-linked branchpoints. High-performance size exclusion chromatography with refractive index detection (HPSEC-RI) revealed the production of inulin with a lower molecular weight compared to other characterized bacterial inulin. LpInu and its inulin product represent novel candidates to be explored for possible food and biomedical applications.