{Reference Type}: Journal Article {Title}: The complete plastome of Acalypha australis (Euphorbiaceae) and its phylogenetic analysis. {Author}: Li H;Ma C;Wang L; {Journal}: Mitochondrial DNA B Resour {Volume}: 9 {Issue}: 5 {Year}: 2024 {Factor}: 0.61 {DOI}: 10.1080/23802359.2023.2294891 {Abstract}: Acalypha australis L. 1753 is a potherb popular among Asian populations and is a traditional herbal medicine. In the current study, the overall genetic diversity of A. australis still needs to be better. Here, we assembled and characterized the complete plastome of A. australis. The plastome is 168,885 bp in length with a large single-copy (LSC) of 94,576 bp, a small single-copy (SSC) of 19,715 bp, and two copies of inverted repeat region (IRs) of 27,297 bp each. The overall GC content is 34.9%. The plastome contains 127 genes, including 83 protein-coding genes, 36 tRNA genes, and eight rRNA genes. Phylogenomic analysis of the representative species of Euphorbiaceae showed that A. australis and A. hispida formed a monophyletic sister clade. The results of this study will support further research on the evolution and conservation of the Euphorbiaceae species; they will benefit pharmaceutical applications and ornamentation of the medicinal plant A. australis.