{Reference Type}: Journal Article
{Title}: Genetically engineered mouse model of HPV16 E6-E7 with vaginal-cervical intraepithelial neoplasia and decreased immunity.
{Author}: Xiurong D;Xin Z;Neng Y;Li D;Yanzhou W;Kaijian L;Zhiqing L;
{Journal}: Heliyon
{Volume}: 10
{Issue}: 10
{Year}: 2024 May 30
{Factor}: 3.776
{DOI}: 10.1016/j.heliyon.2024.e29881
{Abstract}: <B>UNASSIGNED: </B>To construct models of high-risk human papillomavirus (HPV) infection with precancerous lesions or cervical cancer and explore the immune function.<BR><B>UNASSIGNED: </B>Using CRISPR/Cas9, the expression vector HPV16-E6-E7-Rosa26 was microinjected into fertilized eggs of C57BL/6 N mice using homologous recombination, and the F0 generation was obtained for reproduction. Then, the formation of precancerous lesions was promoted via intramuscular injection of estradiol. Presence of precancerous cervical-vaginal intraepithelial lesions, Ki67 and p16 expression levels, and CD8+ T cell proportions in the spleen were evaluated.<BR><B>UNASSIGNED: </B>Two F0 generation mice exhibited correct the homologous recombination. Seven positive mice were identified in the F1 generation. After breeding and mating, 25 homozygous and 11 heterozygous HPV16-E6-E7-engineered mice were obtained from the F2 generation. After estradiol benzoate treatment, the cervical-vaginal epithelium appeared as precancerous lesions with positive Ki67 and p16 expression. The percentage of CD8+ T cells decreased.<BR><B>UNASSIGNED: </B>HPV16-E6-E7-Rosa26 induced low immune function in mice, and provides a good model for the basic research of the mechanisms of action of HPV infection-associated precancerous lesions or cervical cancer.