{Reference Type}: Journal Article
{Title}: Effectiveness of a tissue conditioner containing antifungals in a rat model of denture stomatitis.
{Author}: Moraes GS;Albach T;Sugio CYC;Cachoeira VS;Kiratcz F;Tozetto NM;Pedroso TAA;Campagnoli EB;Claudino M;Dos Santos FA;Neppelenbroek KH;Urban VM;
{Journal}: J Appl Microbiol
{Volume}: 135
{Issue}: 6
{Year}: 2024 Jun 3
{Factor}: 4.059
{DOI}: 10.1093/jambio/lxae123
{Abstract}: <B>OBJECTIVE: </B>This study investigated the effectiveness of a drug-modified tissue conditioner in an animal model of denture stomatitis.<BR><B>RESULTS: </B>Wistar rats wore a Candida albicans-contaminated palatal device for 4 days. Next, nystatin (Nys) or chlorhexidine (Chx) were added to a tissue conditioner in their raw or β-cyclodextrin-complexed (βCD) forms at their minimum inhibitory concentrations. As controls, one group was not subjected to any procedure (NC), one group used sterile devices, one group had denture stomatitis but was not treated (DS), and another had the devices relined with the tissue conditioner without the addition of any drug (Soft). After 4 days of treatment, treatment effectiveness was assessed visually, histologically, and through CFU count, and myeloperoxidase (MPO) and N-acetylglucosaminidase (NAG) assays. Rats from the Soft, Nys, Nys:βCD, and Chx groups presented a significant decrease in the microbial load compared with the untreated group. Treatment groups showed lower MPO and NAG activity compared to the non-treated group.<BR><B>CONCLUSIONS: </B>The addition of antifungals to a soft tissue conditioner can be a promising approach for denture stomatitis treatment.