{Reference Type}: Journal Article
{Title}: Transfection in Primary Cultured Neuronal Cells.
{Author}: Marwick KFM;Hardingham GE;
{Journal}: Methods Mol Biol
{Volume}: 2799
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3830-9_4
{Abstract}: Transfection allows the introduction of foreign nucleic acid into eukaryotic cells. It is an important tool in understanding the roles of NMDARs in neurons. Here we describe using lipofection-mediated transfection to introduce cDNA encoding NMDAR subunits into postmitotic rodent primary cortical neurons maintained in culture.