{Reference Type}: Journal Article
{Title}: Detection of clonal plasma cells in POEMS syndrome using multiparameter flow cytometry.
{Author}: Ishii A;Tsukamoto S;Mimura N;Miyamoto-Nagai Y;Isshiki Y;Matsui S;Nakao S;Shibamiya A;Hino Y;Kayamori K;Oshima-Hasegawa N;Muto T;Takeda Y;Suichi T;Misawa S;Ohwada C;Yokote K;Kuwabara S;Nakaseko C;Takamatsu H;Sakaida E;
{Journal}: Sci Rep
{Volume}: 14
{Issue}: 1
{Year}: 2024 05 6
{Factor}: 4.996
{DOI}: 10.1038/s41598-024-61034-1
{Abstract}: POEMS syndrome (polyneuropathy, organomegaly, endocrinopathy, monoclonal protein [M-protein], and skin changes) is a rare systemic disorder characterized by various symptoms caused by underlying plasma cell (PC) dyscrasia. Detection of monoclonal PCs is mandatory for the diagnosis of POEMS syndrome; however, the usefulness of EuroFlow-based next-generation flow cytometry (EuroFlow-NGF) in POEMS syndrome for detecting monoclonal PCs in bone marrow (BM) and the gating strategy suitable for flow cytometry study of POEMS syndrome remain unknown. We employed EuroFlow-NGF-based single-tube eight-color multiparameter flow cytometry (MM-flow) and established a new gating strategy (POEMS-flow) to detect the monoclonal PCs in POEMS syndrome, gating CD38 broadly from dim to bright and CD45 narrowly from negative to dim compared to MM-flow. MM-flow detected monoclonal PCs in 9/25 (36.0%) cases, including 2/2 immunofixation electrophoresis (IFE)-negative cases (100%). However, POEMS-flow detected monoclonal PCs in 18/25 cases (72.0%), including 2/2 IFE-negative cases (100%). POEMS-flow detected monoclonal PCs with immunophenotypes of CD19- in 17/18 (94.4%). In six cases where post-treatment samples were available, the size of the clones was significantly reduced after the treatment (P = 0.031). POEMS-flow can enhance the identification rate of monoclonal PCs in POEMS syndrome and become a valuable tool for the diagnosis of POEMS syndrome.