{Reference Type}: Journal Article
{Title}: Recent advances in photoaffinity labeling strategies to capture Glycan-Protein interactions.
{Author}: Babulic JL;De León González FV;Capicciotti CJ;
{Journal}: Curr Opin Chem Biol
{Volume}: 80
{Issue}: 0
{Year}: 2024 Jun 3
{Factor}: 8.972
{DOI}: 10.1016/j.cbpa.2024.102456
{Abstract}: Glycans decorate all cells and are critical mediators of cellular processes through recognition by glycan-binding proteins (GBPs). While targeting glycan-protein interactions has great therapeutic potential, these interactions are challenging to study as they are generally transient and exhibit low binding affinities. Glycan-based photo-crosslinkable probes have enabled covalent capture and identification of unknown GBP receptors and glycoconjugate ligands. Here, we review recent progress in photo-crosslinking approaches targeting glycan-mediated interactions. We discuss two prominent emerging strategies: 1) development of photo-crosslinkable oligosaccharide ligands to identify GBP receptors; and 2) cell-surface glyco-engineering to identify glycoconjugate ligands of GBPs. Overall, photoaffinity labeling affords valuable insights into complex glycan-protein networks and is poised to help elucidate the glycan-protein interactome, providing novel targets for therapeutic intervention.