{Reference Type}: Journal Article
{Title}: Identification and functional characterization of a superoxide dismutase (CuZnSOD) from Pinctada fucata martensii.
{Author}: Guo Z;Li C;Liang H;Zhu J;
{Journal}: Fish Shellfish Immunol
{Volume}: 149
{Issue}: 0
{Year}: 2024 Jun 1
{Factor}: 4.622
{DOI}: 10.1016/j.fsi.2024.109599
{Abstract}: Copper/zinc superoxide dismutase (Cu/Zn-SOD) can effectively eliminate reactive oxygen species (ROS)，avoid damage from O2 to the body, and maintain O2 balance. In this study, multi-step high-performance liquid chromatography (HPLC), combined with Mass Spectrometry (MS), was used to isolate and identify Cu/Zn-SOD from the serum of Pinctada fucata martensii (P. f. martensii) and was designated as PmECSOD. With a length of 1864 bp and an open reading frame (ORF) of 1422 bp, the cDNA encodes a 473 amino acid protein. The PmECSOD transcript was detected in multiple tissues by quantitative real-time PCR (qRT-PCR), with its highest expression level being in the gills. Additionally, the temporal expression of PmECSOD mRNA in the hemolymph was highest at 48 h after in vivo stimulation with Escherichia coli and Micrococcus luteus. The results from this study provide a valuable base for further exploration of molluscan innate immunity and immune response.