{Reference Type}: Journal Article
{Title}: Soluble expression of recombinant coagulation factor IX protein using Escherichia coli.
{Author}: Park BH;Yun H;Jeong HJ;
{Journal}: Biochem Biophys Rep
{Volume}: 38
{Issue}: 0
{Year}: 2024 Jul
暂无{DOI}: 10.1016/j.bbrep.2024.101714
{Abstract}: Hemophilia B is a congenital bleeding disorder caused by factor IX (FIX) deficiency. Generation of recombinant FIX (rFIX) is required for detecting a Hemophilia B indicator, anti-FIX antibody. In this study, we described a method for producing recombinant FIX (rFIX) using Escherichia coli. We constructed a FIX-expressing plasmid without a fusion tag protein-encoding gene and produced rFIX as a soluble form within five days. Dose-dependent curve was obtained from ELISA using anti-FIX antibody, indicating that the rFIX can be used as an antigen to detect anti-FIX antibody with high affinity and sensitivity.