{Reference Type}: Journal Article
{Title}: Genome-Driven Discovery of Hygrocins in Streptomyces rapamycinicus.
{Author}: Mahmoud Mohamed MM;Yang Z;Lum KY;Peschel G;Rosenbaum MA;Weber T;Coriani S;Gotfredsen CH;Ding L;
{Journal}: J Nat Prod
{Volume}: 87
{Issue}: 5
{Year}: 2024 May 24
{Factor}: 4.803
{DOI}: 10.1021/acs.jnatprod.3c00767
{Abstract}: Ansamycins, represented by the antituberculosis drug rifamycin, are an important family of natural products. To obtain new ansamycins, Streptomyces rapamycinicus IMET 43975 harboring an ansamycin biosynthetic gene cluster was fermented in a 50 L scale, and subsequent purification work led to the isolation of five known and four new analogues, where hygrocin W (2) belongs to benzoquinonoid ansamycins, and the other three hygrocins, hygrocins X-Z (6-8), are new seco-hygrocins. The structures of ansamycins (1-8) were determined by the analysis of spectroscopic (1D/2D NMR and ECD) and MS spectrometric data. The Baeyer-Villiger enzyme which catalyzed the ester formation in the ansa-ring was confirmed through in vivo CRISPR base editing. The discovery of these compounds further enriches the structural diversity of ansamycins.