{Reference Type}: Journal Article
{Title}: A comprehensive N-glycome map of porcine sperm membrane before and after capacitation.
{Author}: Li Y;Zhai Y;Fu B;He Y;Feng Y;Ma F;Lu H;
{Journal}: Carbohydr Polym
{Volume}: 335
{Issue}: 0
{Year}: 2024 Jul 1
{Factor}: 10.723
{DOI}: 10.1016/j.carbpol.2024.122084
{Abstract}: Mapping the N-glycome of porcine sperm before and after sperm capacitation is important for understanding the rearrangement of glycoconjugates during capacitation. In this work, we characterized the N-glycome on the membranes of 18 pairs of fresh porcine sperm before capacitation and porcine sperm after capacitation by MALDI-MS (Matrix-assisted laser desorption/ionization-mass spectrometry). A total of 377 N-glycans were detected and a comprehensive N-glycome map of porcine sperm membranes before and after capacitation was generated, which presents the largest N-glycome dataset of porcine sperm cell membranes. Statistical analysis revealed a significantly higher level of high mannose glycosylation and a significantly lower level of fucosylation, galactosylation, and α-2,6-NeuAc after capacitation, which is further verified by flow cytometry and lectin blotting. This research reveals new insights into the relationship between N-glycosylation variations and sperm capacitation, including the underlying mechanisms of the capacitation process.