{Reference Type}: Journal Article
{Title}: A lineage-specific protein network at the trypanosome nuclear envelope.
{Author}: Butterfield ER;Obado SO;Scutts SR;Zhang W;Chait BT;Rout MP;Field MC;
{Journal}: Nucleus
{Volume}: 15
{Issue}: 1
{Year}: 2024 Dec
{Factor}: 4.59
{DOI}: 10.1080/19491034.2024.2310452
{Abstract}: The nuclear envelope (NE) separates translation and transcription and is the location of multiple functions, including chromatin organization and nucleocytoplasmic transport. The molecular basis for many of these functions have diverged between eukaryotic lineages. Trypanosoma brucei, a member of the early branching eukaryotic lineage Discoba, highlights many of these, including a distinct lamina and kinetochore composition. Here, we describe a cohort of proteins interacting with both the lamina and NPC, which we term lamina-associated proteins (LAPs). LAPs represent a diverse group of proteins, including two candidate NPC-anchoring pore membrane proteins (POMs) with architecture conserved with S. cerevisiae and H. sapiens, and additional peripheral components of the NPC. While many of the LAPs are Kinetoplastid specific, we also identified broadly conserved proteins, indicating an amalgam of divergence and conservation within the trypanosome NE proteome, highlighting the diversity of nuclear biology across the eukaryotes, increasing our understanding of eukaryotic and NPC evolution.