{Reference Type}: Review {Title}: [Application of raman spectroscopy in the male reproductive system]. {Author}: Zhang LP;Jiao GZ;Liu XY; {Journal}: Zhonghua Nan Ke Xue {Volume}: 29 {Issue}: 5 {Year}: 2023 May 暂无{Abstract}: Current diagnostic techniques for male infertility primarily require invasive testing of sperm. Clinically, there is a need for a reliable, non-invasive analysis method that provides precise information about sperm quality without compromising sperm cell integrity. Raman spectroscopy, utilizing the inelastic scattering spectra of light, offers a rapid, simple, repeatable, and non-destructive approach for both qualitative and quantitative analysis, gaining widespread application in medicine. This paper reviews the fundamental characteristics of Raman spectroscopy and its applications in the male reproductive system.