{Reference Type}: Journal Article
{Title}: Full-length αIIbβ3 cryo-EM structure reveals intact integrin initiate-activation intrinsic architecture.
{Author}: Huo T;Wu H;Moussa Z;Sen M;Dalton V;Wang Z;
{Journal}: Structure
{Volume}: 32
{Issue}: 7
{Year}: 2024 Jul 11
{Factor}: 5.871
{DOI}: 10.1016/j.str.2024.03.006
{Abstract}: Integrin αIIbβ3 is the key receptor regulating platelet retraction and accumulation and a proven drug-target for antithrombotic therapies. Here we resolve the cryo-EM structures of the full-length αIIbβ3, which covers three distinct states along the activation pathway. Firstly, we obtain the αIIbβ3 structure at 3 Å resolution in the inactive state, revealing the overall topology of the heterodimer with the transmembrane (TM) helices and the ligand-binding domain tucked in a specific angle proximity to the TM region. After the addition of a Mn2+ agonist, we resolve two coexisting structures representing two new states between inactive and active state. Our structures show conformational changes of the αIIbβ3 activating trajectory and a unique twisting of the integrin legs, which is required for platelets accumulation. Our structure provides direct structural evidence for how the lower legs are involved in full-length integrin activation mechanisms and offers a new strategy to target the αIIbβ3 lower leg.