{Reference Type}: Journal Article
{Title}: Catechol-O-methyltransferase and monoamine oxidase B inhibitory activities of Australian bee pollen.
{Author}: Watanabe C;Yanagihara A;Miyata R;Mitsui T;Honda C;Fujinami D;Kumazawa S;
{Journal}: Biosci Biotechnol Biochem
{Volume}: 88
{Issue}: 6
{Year}: 2024 May 22
{Factor}: 2.337
{DOI}: 10.1093/bbb/zbae041
{Abstract}: Bee pollen is an apicultural product collected by honeybees from flower stamens and used as a functional food worldwide. In the present study, we aim to elucidate the functions of Australian bee pollen. Australian bee pollen extracts and their main components were tested for catechol-O-methyltransferase (COMT) and monoamine oxidase B (MAOB) inhibitory activities. These enzymes are key neurotransmitters involved in Parkinson's disease and depression. Myricetin (5), tricetin (6), and luteolin (7) exhibited high COMT inhibitory activities (half maximal inhibitory concentration [IC50] = 23.3, 13.8, and 47.4 µM, respectively). In contrast, 5, 7, and annulatin (8) exhibited MAOB inhibitory activities (IC50 = 89.7, 32.8, and 153 µM, respectively). Quantitative analysis via high-performance liquid chromatography revealed that 5 was abundant in Australian bee pollen extracts. Our findings suggest that 5 contributes to the COMT and MAOB inhibitory activities of Australian bee pollen.