{Reference Type}: Journal Article
{Title}: Using the DNA Integrity Number to Analyze DNA Quality in Specimens Collected from Liquid-Based Cytology after Fine-Needle Aspiration of Breast Tumors and Lesions.
{Author}: Hoshino A;Oana Y;Ohi Y;Maeda Y;Omori M;Takada Y;Ikeda T;Sotome K;Maeda H;Yanagisawa T;Takeuchi O;Kuronuma S;Sangai T;Shibahara Y;Murakumo Y;Saegusa M;Kanomata N;Nagasawa S;Yamaguchi R;Yoshida M;Kozuka Y;Matsumoto H;Tsugawa K;Maeda I; ;
{Journal}: Acta Cytol
{Volume}: 68
{Issue}: 2
{Year}: 2024 Mar 29
{Factor}: 3
{DOI}: 10.1159/000538071
{Abstract}: <B>BACKGROUND: </B>Cancer genome analysis using next-generation sequencing requires adequate and high-quality DNA samples. Genomic analyses were conventionally performed using formalin-fixed paraffin-embedded sections rather than cytology samples such as cell block or smear specimens. Specimens collected from liquid-based cytology (LBC) have the potential to be sources of high-quality DNA suitable for genetic analysis even after long-term storage.<BR><B>METHODS: </B>We collected breast tumor/lesion fractions from 92 residual LBC specimens using fine-needle aspiration (FNA) biopsy, including breast carcinoma (1 invasive carcinoma and 4 ductal carcinomas in situ), papillomatous lesion (5 intraductal papillomas), and fibroepithelial lesion (19 phyllodes tumors and 53 fibroadenomas) samples, and others (1 ductal adenoma, 1 hamartoma, 1 fibrocystic disease, and 7 unknown). DNA was extracted from all samples and subjected to DNA integrity number (DIN) score analysis.<BR><B>RESULTS: </B>Average DIN score collected from 92 LBC specimens was significantly higher score. In addition, high-quality DNA with high DIN values (7.39 ± 0.80) was successfully extracted more than 12 months after storage of residual LBC specimens.<BR><B>CONCLUSIONS: </B>Residual LBC specimens collected from FNA of the breast were verified to carry high-quality DNA and could serve as an alternate source for genetic analysis.