{Reference Type}: Journal Article
{Title}: Diagnosis of abdominal tuberculosis: Detection of mycobacterial CFP-10 and HspX proteins by gold nanoparticle-PCR amplified immunoassay.
{Author}: Dahiya B;Mor P;Rais A;Prasad T;Sheoran A;Sheoran R;Sharma S;Seth MK;Srivastava SK;Mehta PK;
{Journal}: J Microbiol Methods
{Volume}: 220
{Issue}: 0
{Year}: 2024 May 27
{Factor}: 2.622
{DOI}: 10.1016/j.mimet.2024.106925
{Abstract}: Attempts were made to improve the efficacy of PCR amplified immunoassay (I-PCR) for diagnosing abdominal TB cases by utilizing the gold nanoparticle (AuNP)-based I-PCR, where AuNPs were functionalized with detection antibodies/oligonucleotides that exhibited 84.3% sensitivity and 95.1% specificity. This assay would improve the ongoing algorithms used in abdominal TB diagnosis.