{Reference Type}: Journal Article
{Title}: Construction of recombinant fluorescent LSDV for high-throughput screening of antiviral drugs.
{Author}: Wang J;Ji J;Zhong Y;Meng W;Wan S;Ding X;Chen Z;Wu W;Jia K;Li S;
{Journal}: Vet Res
{Volume}: 55
{Issue}: 1
{Year}: 2024 Mar 16
{Factor}: 3.829
{DOI}: 10.1186/s13567-024-01281-2
{Abstract}: Lumpy skin disease virus (LSDV) infection is a major socio-economic issue that seriously threatens the global cattle-farming industry. Here, a recombinant virus LSDV-ΔTK/EGFP, expressing enhanced green fluorescent protein (EGFP), was constructed with a homologous recombination system and applied to the high-throughput screening of antiviral drugs. LSDV-ΔTK/EGFP replicates in various kidney cell lines, consistent with wild-type LSDV. The cytopathic effect, viral particle morphology, and growth performance of LSDV-ΔTK/EGFP are consistent with those of wild-type LSDV. High-throughput screening allowed to identify several molecules that inhibit LSDV-ΔTK/EGFP replication. The strong inhibitory effect of theaflavin on LSDV was identified when 100 antiviral drugs were screened in vitro. An infection time analysis showed that theaflavin plays a role in the entry of LSDV into cells and in subsequent viral replication stages. The development of this recombinant virus will contribute to the development of LSDV-directed antiviral drugs and the study of viral replication and mechanisms of action.