{Reference Type}: Journal Article
{Title}: Identification of plb1 mutation that extends longevity via activating Sty1 MAPK in Schizosaccharomyces pombe.
{Author}: Maekawa Y;Matsui K;Okamoto K;Shimasaki T;Ohtsuka H;Tani M;Ihara K;Aiba H;
{Journal}: Mol Genet Genomics
{Volume}: 299
{Issue}: 1
{Year}: 2024 Feb 29
{Factor}: 2.98
{DOI}: 10.1007/s00438-024-02107-8
{Abstract}: To understand the lifespan of higher organisms, including humans, it is important to understand lifespan at the cellular level as a prerequisite. So, fission yeast is a good model organism for the study of lifespan. To identify the novel factors involved in longevity, we are conducting a large-scale screening of long-lived mutant strains that extend chronological lifespan (cell survival in the stationary phase) using fission yeast. One of the newly acquired long-lived mutant strains (No.98 mutant) was selected for analysis and found that the long-lived phenotype was due to a missense mutation (92Phe → Ile) in the plb1+ gene. plb1+ gene in fission yeast is a nonessential gene encoding a homolog of phospholipase B, but its functions under normal growth conditions, as well as phospholipase B activity, remain unresolved. Our analysis of the No.98 mutant revealed that the plb1 mutation reduces the integrity of the cellular membrane and cell wall and activates Sty1 via phosphorylation.