{Reference Type}: Journal Article
{Title}: Synchronous force and Ca2+ measurements for repeated characterization of excitation-contraction coupling in human myocardium.
{Author}: Sun Z;Lu K;Kamla C;Kameritsch P;Seidel T;Dendorfer A;
{Journal}: Commun Biol
{Volume}: 7
{Issue}: 1
{Year}: 2024 Feb 22
{Factor}: 6.548
{DOI}: 10.1038/s42003-024-05886-3
{Abstract}: Dysfunctional Ca2+ signaling affects the myocardial systole and diastole, may trigger arrhythmia and cause transcriptomic and proteomic modifications in heart failure. Thus, synchronous real-time measurement of Ca2+ and force is essential to investigate the relationship between contractility and Ca2+ signaling and the alteration of excitation-contraction coupling (ECC) in human failing myocardium. Here, we present a method for synchronized acquisition of intracellular Ca2+ and contraction force in long-term cultivated slices of human failing myocardium. Synchronous time series of contraction force and intracellular Ca2+ were used to calculate force-calcium loops and to analyze the dynamic alterations of ECC in response to various pacing frequencies, post-pause potentiation, high mechanical preload and pharmacological interventions in human failing myocardium. We provide an approach to simultaneously and repeatedly investigate alterations of contractility and Ca2+ signals in long-term cultured myocardium, which will allow detecting the effects of electrophysiological or pharmacological interventions on human myocardial ECC.