{Reference Type}: Journal Article
{Title}: Biomanufacture of L-homoserine lactone building block: A strategy for preparing γ-substituted L-amino acids by modular reaction.
{Author}: Wang Y;Wu M;Zheng H;Wu D;Yao P;Li W;Jin K;Yu X;
{Journal}: Enzyme Microb Technol
{Volume}: 176
{Issue}: 0
{Year}: 2024 May 7
{Factor}: 3.705
{DOI}: 10.1016/j.enzmictec.2024.110411
{Abstract}: A strain high-performance of esterase producing bacteria was screened from soil, which could selectively hydrolyze D-homoserine lactone from its racemate to achieve the resolution of L- homoserine lactone with more than 99% e.e. in 48% yield. L-homoserine lactone building block was then converted to L-α-amino-γ-bromobutyronic acid chiral blocks, which reacted with various nucleophilic reagent modules could to be applied to prepare L-γ- substituted α-amino acids such as L-selenomethionine, L-methionine, L-glufosinate and L-selenocystine. Its advantages included high selectivity of biocatalytic resolution reactions, high optical purity of products, racemic recycle of D-substrates and modular reaction, which simplified the production process of these products and highlighted the power of biological manufacturing.