{Reference Type}: Comparative Study
{Title}: Comparison between hangeshashinto and dexamethasone for IL-1α and β-defensin 1 production by human oral keratinocytes.
{Author}: Hato H;Kaneko A;Maeda C;Sakata KI;Ono Y;Mizukami Y;Kono T;Kitagawa Y;
{Journal}: J Oral Biosci
{Volume}: 66
{Issue}: 1
{Year}: 2024 Mar 24
暂无{DOI}: 10.1016/j.job.2024.01.007
{Abstract}: <B>OBJECTIVE: </B>Human β-defensin 1 (hBD-1) is a antimicrobial peptide that is constantly secreted by oral tissues. Hangeshashinto (HST), a traditional Japanese medicine, has been reported to be effective against stomatitis. This study aimed to clarify the profile of HST by comparing the system of production of interleukin-1α (IL-1α) and hBD-1 in human oral mucosal epithelial cells with dexamethasone (DEX), a steroid used for the treatment of stomatitis.<BR><B>METHODS: </B>Human oral keratinocytes (HOK) were treated with HST, DEX, or HST components (baicalein, baicalin, berberine, and glycyrrhizin) for 24 h, and subsequently cultured for 24 h with or without Pam3CSK4 or lipopolysaccharide (LPS). The cell supernatants, total RNA, and intracellular proteins were collected, and changes in IL-1α and hBD-1 protein production and gene expression were evaluated using ELISA and RT-PCR. The phosphorylation of NF-kB and the cell proliferative ability of HOK were evaluated by western blotting and XTT assay, respectively.<BR><B>RESULTS: </B>DEX (0.01-10 μM) significantly suppressed IL-1α and hBD-1 production induced by either Pam3CSK4 or LPS, and also decreased cell growth. In contrast, HST inhibited Pam3CSK4- and LPS-induced IL-1α production at a concentration range of 12.5-100 μg/mL without affecting the cell proliferative capacity and hBD-1 production of HOK. Baicalein and baicalin, which are flavonoid ingredients of HST, showed anti-IL-1α production.<BR><B>CONCLUSIONS: </B>HST may be useful as a therapeutic agent for stomatitis and other inflammatory diseases of the oral cavity.