{Reference Type}: Journal Article {Title}: Review of the endemic Neotropical genus Agenamyia Albuquerque (Diptera: Muscidae) with the description of new species from South America. {Author}: Carvalho CJB;Wolff M;Haseyama KLF;Fogaa JM; {Journal}: Zootaxa {Volume}: 5346 {Issue}: 5 {Year}: 2023 Sep 21 {Factor}: 1.026 {DOI}: 10.11646/zootaxa.5346.5.2 {Abstract}: Agenamyia Albuquerque, an endemic neotropical genus with two species, has been revised to include three new species: Agenamyia colombiana de Carvalho, Wolff & Fogaa sp. nov. from Envigado; Agenamyia maculata de Carvalho, Wolff & Fogaa sp. nov. from El Retiro; and Agenamyia timida de Carvalho, Wolff & Fogaa sp. nov. from Sopetrn, all localities in Antioquia, Colombia. This review presents an identification key for all species, including color images and illustrations of male and female terminalia. The distribution of all species is mapped and discussed.