{Reference Type}: Journal Article
{Title}: IL-4-secreting CD40L+ MAIT cells support antibody production in the peripheral blood of Heonch-Schönlein purpura patients.
{Author}: Liu M;Yang Z;Wu Q;Yang Y;Zhao D;Cheng Q;Li Y;Liu G;Zhao C;Pan J;Zhang Y;Deng F;Jin T;
{Journal}: Inflamm Res
{Volume}: 73
{Issue}: 1
{Year}: 2024 Jan 26
{Factor}: 6.986
{DOI}: 10.1007/s00011-023-01816-x
{Abstract}: <B>OBJECTIVE: </B>Here, we explored the phenotype and function of MAIT cells in the peripheral blood of patients with HSP.<BR><B>METHODS: </B>Blood samples from HSP patients and HDs were assessed by flow cytometry and single-cell RNA sequencing to analyze the proportion, phenotype, and function of MAIT cells. Th-cytokines in the serum of HSP patients were analyzed by CBA. IgA in cocultured supernatant was detected by CBA to analyze antibody production by B cells.<BR><B>RESULTS: </B>The percentage of MAIT cells in HSP patients was significantly reduced compared with that in HDs. Genes related to T cell activation and effector were up-regulated in HSP MAIT cells, indicating a more activated phenotype. In addition, HSP MAIT cells displayed a Th2-like profile with the capacity to produce more IL-4 and IL-5, and IL-4 was correlated with IgA levels in the serum of HSP patients. Furthermore, CD40L was up-regulated in HSP MAIT cells, and CD40L+ MAIT cells showed an increased ability to produce IL-4 and to enhance IgA production by B cells.<BR><B>CONCLUSIONS: </B>Our data demonstrate that MAIT cells in HSP patients exhibit an activated phenotype. The enhanced IL-4 production and CD40L expression of MAIT cells in HSP patients could take part in the pathogenesis of HSP.