{Reference Type}: Journal Article
{Title}: Antibiotic that inhibits trans-translation blocks binding of EF-Tu to tmRNA but not to tRNA.
{Author}: Marathe N;Nguyen HA;Alumasa JN;Kuzmishin Nagy AB;Vazquez M;Dunham CM;Keiler KC;
{Journal}: mBio
{Volume}: 14
{Issue}: 5
{Year}: 2023 Oct 31
暂无{DOI}: 10.1128/mbio.01461-23
{Abstract}: <B>OBJECTIVE: </B>Elongation factor thermo-unstable (EF-Tu) is a universally conserved translation factor that mediates productive interactions between tRNAs and the ribosome. In bacteria, EF-Tu also delivers transfer-messenger RNA (tmRNA)-SmpB to the ribosome during trans-translation. We report the first small molecule, KKL-55, that specifically inhibits EF-Tu activity in trans-translation without affecting its activity in normal translation. KKL-55 has broad-spectrum antibiotic activity, suggesting that compounds targeted to the tmRNA-binding interface of EF-Tu could be developed into new antibiotics to treat drug-resistant infections.