{Reference Type}: Review
{Title}: A Rare Case of Falsely Elevated High-Sensitivity Cardiac Troponin T due to Antibody Interference and a Literature Review.
{Author}: Cheng F;Chen J;Wu BL;Yang X;Huang YS;
{Journal}: Clin Lab
{Volume}: 69
{Issue}: 8
{Year}: 2023 Aug 1
{Factor}: 1.053
{DOI}: 10.7754/Clin.Lab.2023.230230
{Abstract}: <B>BACKGROUND: </B>This study aimed to demonstrate potential measures to discern interference and establish an algorithm for identifying interference in an hs-cTnT assay with a clinical discrepancy.<BR><B>METHODS: </B>Laboratory error and disease factors were first excluded. Then, a serial dilution test, polyethylene glycol (PEG) precipitation, and heterophile antibody blocking reagents (HBRs) were performed to confirm the interference substance. In addition, high-speed centrifugation and western blot were tried.<BR><B>RESULTS: </B>The sample diluted linearly (R2 = 0.9966). However, there was a dramatic reduction in the concentration of hs-cTnT concentration to a normal level after both PEG precipitation and HBR1 treatment. The results were inconclusive in high-speed centrifugation and western blot assay.<BR><B>CONCLUSIONS: </B>This study elucidated a heterophile antibody interference in measurement of hs-cTnT alone on a Roche analyzer.