{Reference Type}: English Abstract
{Title}: [Effect of LncRNA GATA3-AS1 Targeting MiR-515-5p on Cell Proliferation and Apoptosis in Childhood Acute Lymphoblastic Leukemia Cells].
{Author}: Qin Y;Yun XX;Zheng ZM;Xu Q;Zuo LM;
{Journal}: Zhongguo Shi Yan Xue Ye Xue Za Zhi
{Volume}: 31
{Issue}: 4
{Year}: 2023 Aug
暂无{DOI}: 10.19746/j.cnki.issn.1009-2137.2023.04.016
{Abstract}: <B>OBJECTIVE: </B>To investigate the effects of long non-coding RNA (lncRNA) GATA3 antisense RNA 1 (GATA3-AS1) targeting miR-515-5p on the proliferation and apoptosis of childhood acute lymphoblastic leukemia (ALL) cells.<BR><B>METHODS: </B>RT-qPCR was used to determine the expression of GATA3-AS1 and miR-515-5p in the plasma of controls and ALL children. Human ALL cells Jurkat were divided into si-GATA3-AS1, si-NC, miR-NC, miR-515-5p, si-GATA3-AS1+anti-miR-NC and si-GATA3-AS1+anti-miR-515-5p groups. CCK-8 assay was used to detect the cell proliferation, and flow cytometry was used to detect the cell apoptosis. The targeting relationship between GATA3-AS1 and miR-515-5p was determined by dual-luciferase reporter assay.<BR><B>RESULTS: </B>The expression level of GATA3-AS1 in the plasma of ALL children was significantly higher than that of controls (P <0.001), while the expression level of miR-515-5p was significantly lower than that of controls (P <0.001). Compared with the si-NC group, the cell inhibition rate, apoptosis rate, and miR-515-5p expression level in si-GATA3-AS1 group were significantly increased (P <0.001). Compared with the miR-NC group, the cell inhibition rate and apoptosis rate in miR-515-5p group were significantly increased (P <0.001). GATA3-AS1 could directly and specifically bind to miR-515-5p. Compared with the si-GATA3-AS1+anti-miR-NC group, the cell inhibition rate and apoptosis rate in si-GATA3-AS1+anti-miR-515-5p group were significantly decreased (P <0.001).<BR><B>CONCLUSIONS: </B>Down-regulation of GATA3-AS1 can inhibit proliferation and induce apoptosis of childhood ALL cells by targeting up-regulation of miR-515-5p expression.<BR><B>UNASSIGNED: </B>lncRNA GATA3-AS1靶向miR-515-5p对儿童急性淋巴细胞 白血病细胞增殖和凋亡的影响.<BR><B>UNASSIGNED: </B>探讨长链非编码RNA（lncRNA）GATA3反义RNA1（GATA3-AS1）靶向miR-515-5p 对儿童急性淋巴细胞白血病（ALL）细胞增殖和凋亡的影响。.<BR><B>UNASSIGNED: </B>RT-qPCR检测对照者和ALL患儿血浆中GATA3-AS1和miR-515-5p的表达水平。将人ALL细胞Jurkat分为si-GATA3-AS1、si-NC、miR-NC、miR-515-5p、si-GATA3-AS1+anti-miR-NC和si-GATA3-AS1 +anti-miR-515-5p共6组。CCK-8法检测细胞增殖，流式细胞术分析细胞凋亡，双荧光素酶报告实验用于确定GATA3-AS1和miR-515-5p的靶向关系。.<BR><B>UNASSIGNED: </B>ALL患儿血浆中GATA3-AS1表达水平显著高于对照者（P <0.001 ），miR-515-5p表达水平显著低于对照者（P <0.001）。与si-NC组比较，si-GATA3-AS1组细胞抑制率、凋亡率、miR-515-5p表达水平显著升高（P <0.001）。与miR-NC组比较，miR-515-5p组细胞抑制率、凋亡率显著升高（P <0.001 ）。GATA3-AS1与miR-515-5p直接特异性结合。与si-GATA3-AS1+anti-miR-NC组比较，si-GATA3-AS1+ anti-miR-515-5p组细胞抑制率、凋亡率显著降低（P <0.001）。.<BR><B>UNASSIGNED: </B>下调GATA3-AS1可通过靶向上调miR-515-5p的表达抑制儿童ALL细胞增殖，并诱导细胞凋亡。.