{Reference Type}: Journal Article
{Title}: Increasing the coverage of the N-terminome with LysN amino terminal enrichment (LATE).
{Author}: Hanna R;Rozenberg A;Lavy T;Kleifeld O;
{Journal}: Methods Enzymol
{Volume}: 686
{Issue}: 0
{Year}: 2023
{Factor}: 1.682
{DOI}: 10.1016/bs.mie.2023.04.003
{Abstract}: The field of N-terminomics has been advancing with the development of novel methods that provide a comprehensive and unbiased view of the N-terminome. Negative selection N-terminomics enables the identification of free and naturally modified protein N-termini. Here, we present a streamlined protocol that combines two negative selection N-terminomics methods, LATE and HYTANE, to increase N-terminome coverage by 1.5-fold compared to using a single methodology. Our protocol includes sample preparation and data analysis of both methods and can be applied to studying the N-terminome of diverse samples. The suggested approach enables researchers to achieve a more detailed and accurate understanding of the N-terminome.