{Reference Type}: Journal Article {Title}: Analysis of long-term spatio-temporal wetland change reveals the complex nature of habitat alterations - A case study from the Czech Republic 1842-2017. {Author}: Toman V;Skaloš J;Özman KO; {Journal}: Sci Total Environ {Volume}: 894 {Issue}: 0 {Year}: 2023 Oct 10 {Factor}: 10.753 {DOI}: 10.1016/j.scitotenv.2023.164769 {Abstract}: Wetlands fulfil a number of functions in the landscape, especially non-productive ones. Information on landscape and biotope changes is important not only from a theoretical point of view for understanding the forces and pressures that cause changes in the landscape, but also from a practical point of view, as we can take inspiration from history when planning the landscape. The main goal of this study is to analyse the dynamics and trajectories of changes in wetlands, including testing the influence of the main natural conditions (climate, geomorphology) on their changes, for a large area of 141 cadastral territories (1315 km2), which will allow the results to be sufficiently generalized. The results of our study confirmed the global trend of rapid wetland loss, with almost three quarters of wetlands disappearing, mostly on arable land (37 %). The results of the study are of great importance in the field of the ecology of landscapes and wetlands, both in the national and international context, not only because they make it possible to understand the regularities and forces that affect changes in wetlands and landscapes, but also have significance due to the methodology. The specific methodology and procedure are based on the application of advanced GIS functions (Union and Intersect functions) to identify the location and area of individual change dynamics and types of wetland (new, extinct, continuous), using accurate old large-scale maps and aerial photographs. The proposed and tested methodological procedure can generally be used for wetlands in other locations, but also for studying the dynamics of changes and trajectories of other biotopes in the landscape. The greatest potential for using the results of this work in the field of environmental protection is the possibility of using the places of extinct wetlands for their restoration.