{Reference Type}: Journal Article
{Title}: Introduction of an electrochemical point-of-care assay for quantitative determination of paracetamol in finger-prick capillary whole blood samples.
{Author}: Kujala J;Wester N;Lohela TJ;Kurkela M;Backman JT;Mikladal B;Laurila T;Koskinen J;Lilius TO;Kalso EA;
{Journal}: Br J Clin Pharmacol
{Volume}: 89
{Issue}: 9
{Year}: 2023 09 22
{Factor}: 3.716
{DOI}: 10.1111/bcp.15794
{Abstract}: Measuring venous plasma paracetamol concentrations is time- and resource-consuming. We aimed to validate a novel electrochemical point-of-care (POC) assay for rapid paracetamol concentration determinations.<BR>Twelve healthy volunteers received 1 g oral paracetamol, and its concentrations were analysed 10 times over 12 h for capillary whole blood (POC), venous plasma (high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS)), and dried capillary blood (HPLC-MS/MS).<BR>At concentrations >30 μM, POC showed upward biases of 20% (95% limits of agreement [LOA] -22 to 62) and 7% (95% LOA -23 to 38) compared with venous plasma and capillary blood HPLC-MS/MS, respectively. There were no significant differences between mean concentrations for the paracetamol elimination phase.<BR>Upward biases in POC compared with venous plasma HPLC-MS/MS were likely due to higher paracetamol concentrations in capillary blood than in venous plasma and to faulty individual sensors. The novel POC method is a promising tool for paracetamol concentration analysis.