{Reference Type}: Journal Article
{Title}: STAT3 signaling in B cells controls germinal center zone organization and recycling.
{Author}: Fike AJ;Chodisetti SB;Wright NE;Bricker KN;Domeier PP;Maienschein-Cline M;Rosenfeld AM;Luckenbill SA;Weber JL;Choi NM;Luning Prak ET;Mandal M;Clark MR;Rahman ZSM;
{Journal}: Cell Rep
{Volume}: 42
{Issue}: 5
{Year}: 2023 05 30
暂无{DOI}: 10.1016/j.celrep.2023.112512
{Abstract}: Germinal centers (GCs), sites of antibody affinity maturation, are organized into dark (DZ) and light (LZ) zones. Here, we show a B cell-intrinsic role for signal transducer and activator of transcription 3 (STAT3) in GC DZ and LZ organization. Altered zonal organization of STAT3-deficient GCs dampens development of long-lived plasma cells (LL-PCs) but increases memory B cells (MBCs). In an abundant antigenic environment, achieved here by prime-boost immunization, STAT3 is not required for GC initiation, maintenance, or proliferation but is important for sustaining GC zonal organization by regulating GC B cell recycling. Th cell-derived signals drive STAT3 tyrosine 705 and serine 727 phosphorylation in LZ B cells, regulating their recycling into the DZ. RNA sequencing (RNA-seq) and chromatin immunoprecipitation sequencing (ChIP-seq) analyses identified STAT3 regulated genes that are critical for LZ cell recycling and transiting through DZ proliferation and differentiation phases. Thus, STAT3 signaling in B cells controls GC zone organization and recycling, and GC egress of PCs, but negatively regulates MBC output.