{Reference Type}: Journal Article
{Title}: Elucidating cellular interactome of chikungunya virus identifies host dependency factors.
{Author}: Yin P;Jian X;Liu Y;Liu Y;Lv L;Cui H;Zhang L;
{Journal}: Virol Sin
{Volume}: 38
{Issue}: 4
{Year}: 2023 Aug 12
{Factor}: 6.947
{DOI}: 10.1016/j.virs.2023.05.007
{Abstract}: Chikungunya virus (CHIKV) is a re-emerging mosquito-transmitted RNA virus causing joint and muscle pain. To better understand how CHIKV rewires the host cell and usurps host cell functions, we generated a systematic CHIKV-human protein-protein interaction map and revealed several novel connections that will inform further mechanistic studies. One of these novel interactions, between the viral protein E1 and STIP1 homology and U-box containing protein 1 (STUB1), was found to mediate ubiquitination of E1 and degrade E1 through the proteasome. Capsid associated with G3BP1, G3BP2 and AAA+ ​ATPase valosin-containing protein (VCP). Furthermore, VCP inhibitors blocked CHIKV infection, suggesting VCP could serve as a therapeutic target. Further work is required to fully understand the functional consequences of these interactions. Given that CHIKV proteins are conserved across alphaviruses, many virus-host protein-protein interactions identified in this study might also exist in other alphaviruses. Construction of interactome of CHIKV provides the basis for further studying the function of alphavirus biology.