{Reference Type}: Journal Article
{Title}: cAMP-EPAC-PKCε-RIM1α signaling regulates presynaptic long-term potentiation and motor learning.
{Author}: Wang XT;Zhou L;Dong BB;Xu FX;Wang DJ;Shen EW;Cai XY;Wang Y;Wang N;Ji SJ;Chen W;Schonewille M;Zhu JJ;De Zeeuw CI;Shen Y;
{Journal}: Elife
{Volume}: 12
{Issue}: 0
{Year}: 2023 04 26
{Factor}: 8.713
{DOI}: 10.7554/eLife.80875
{Abstract}: The cerebellum is involved in learning of fine motor skills, yet whether presynaptic plasticity contributes to such learning remains elusive. Here, we report that the EPAC-PKCε module has a critical role in a presynaptic form of long-term potentiation in the cerebellum and motor behavior in mice. Presynaptic cAMP-EPAC-PKCε signaling cascade induces a previously unidentified threonine phosphorylation of RIM1α, and thereby initiates the assembly of the Rab3A-RIM1α-Munc13-1 tripartite complex that facilitates docking and release of synaptic vesicles. Granule cell-specific blocking of EPAC-PKCε signaling abolishes presynaptic long-term potentiation at the parallel fiber to Purkinje cell synapses and impairs basic performance and learning of cerebellar motor behavior. These results unveil a functional relevance of presynaptic plasticity that is regulated through a novel signaling cascade, thereby enriching the spectrum of cerebellar learning mechanisms.